Fig. 3
From: Detecting eukaryotic microbiota with single-cell sensitivity in human tissue
Sensitivity of 18S rRNA gene sequencing and HiSeq X Ten sequencing. Genome-copy detection limit determination of P. falciparum, T. gondii, and S. cerevisiae in positive control samples using a dual-index sequencing approach. a Representation of the percentage of 18S reads per eukaryotic positive control signal as measured. b Normalized representation of the absolute comparative strength of each eukaryotic signal after accounting for a different total eukaryotic signal strength in each of the positive control samples using 18S amplification. A value of 100,000 is representative of the human signal strength in each sample; P. falciparum, T. gondii, and S. cerevisiae signal strength values were calculated based upon the total remaining percentage of human reads in each positive control. c HiSeq X Ten genome-copy detection limit determination of P. falciparum, T. gondii, and S. cerevisiae