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Fig. 7 | Microbiome

Fig. 7

From: The tick endosymbiont Candidatus Midichloria mitochondrii and selenoproteins are essential for the growth of Rickettsia parkeri in the Gulf Coast tick vector

Fig. 7

The impact of selenoprotein silencing on tick symbionts in R. parkeri (Rp+)-infected A. maculatum. The selenogene-silenced tick tissues (from Fig. 5) on day 5 post-microinjection of dsRNA–SELENOM, dsRNA–SELENOO, or dsRNA–SELENOS were used to determine the total bacterial load (BL) and Rp load in SELENOM- (a, b), SELENOO- (c, d), and SELENOS-silenced ticks (e, f). The qRT-PCR assay described in Figs. 1 and 2 was used to estimate the total bacterial load and Rp load per tick GAPDH. The p value is provided to compare the statistical significance between the selenogene-silenced ticks and the control ticks. A p value of < 0.05 was considered statistically significant

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