Fig. 6From: The tick endosymbiont Candidatus Midichloria mitochondrii and selenoproteins are essential for the growth of Rickettsia parkeri in the Gulf Coast tick vectorThe impact of tick selenoprotein gene silencing on total bacterial and R. parkeri loads in the tick. The selenogene-silenced tick tissues (from Fig. 5) 5 days after microinjections of dsRNA–SELENOM, dsRNA–SELENOO, or dsRNA–SELENOS were used to estimate the total bacterial load (BL) and R. parkeri (Rp) load in the SELENOM- (a, b), SELENOO- (c, d), and SELENOS-silenced ticks (e, f). The qRT-PCR assay described in Figs. 1 and 2 was used to estimate the total bacterial load and Rp load per tick GAPDH. The p value is provided to compare statistical significance between the selenogene-silenced ticks and the control ticks. A p value of < 0.05 was considered statistically significantBack to article page