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Fig. 4 | Microbiome

Fig. 4

From: The tick endosymbiont Candidatus Midichloria mitochondrii and selenoproteins are essential for the growth of Rickettsia parkeri in the Gulf Coast tick vector

Fig. 4

Differentially expressed tick selenogenes in R. parkeri-infected (Rp+) adult female ticks on day 5 after feeding. The Rp+ and R. parkeri-free adult female ticks that fed on sheep (in Fig. 1) were removed from the host on day 5 post-infestation and then dissected for tissue collection (midguts, salivary glands, and ovaries). Tick midguts and salivary glands isolated from single ticks and ovarian tissues were pooled from five individually-dissected Rp+ and Rp ticks. Quantitative reverse transcriptase PCR (qRT-PCR) was used to determine the transcriptional expression levels of the tick selenogenes. Differential gene expression was estimated in a tick midguts, b salivary glands, and c ovarian tissues. The expression levels in the Rp tick tissues were set to 1, as represented by dashed lines. eEFSec: selenocysteine elongation factor, SELENOM: selenoprotein M, SELENOK: selenoprotein K, SELENOS: selenoprotein S, SELENOO: selenoprotein O, TrxR: thioredoxin reductase, GST: glutathione S-transferase, SELENON: selenoprotein N, SELENOX: selenoprotein X, SELENOT: selenoprotein T

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