Fine metagenomic profile of the Mediterranean stratified and mixed water columns revealed by assembly and recruitment

Table 1 Summary statistics of the sampling, sequencing, and assembly parameters

	MedWinter-JAN2015-20m	MedWinter-JAN2015-80m	Med-OCT2015-15m	Med-OCT2015-30m	Med-OCT2015-45m	Med-OCT2015-60m	Med-OCT2015-75m	Med-OCT2015-90m	Med-OCT2015-1000m
Sampling parameters
Sampling data	1/27/2015		10/15/2015						10/16/2015
Collection depth (m)	20	80	15	30	45	60	75	90	1000
Sea bottom depth (m)	200		2647
Size fraction (μm)	5–0.22		5–0.22
Latitude (N)	38.06851		37.35361
Longitude (W)	0.231994		0.286194
Environmental parameters
Temperature (°C)	14.50	14.40	22.90	18.40	15.80	14.50	14.00	13.80	13.10
Chlorophyll (mg/m³)	0.46	0.21	0.10	0.24	0.78	0.36	0.26	0.08	0.01
Oxygen (mg/L)	9.59	9.42	7.09	8.77	9.00	7.66	7.16	6.88	6.10
TOC (mgC/L)	1.23	1.03	2.43	2.17	1.46	1.43	1.36	1.35	0.84
PO₄³⁻ (μM)	0.12	0.08	0.06	0.07	0.10	0.08	0.12	0.22	0.39
Total P (μM)	0.15	0.12	0.10	0.12	0.14	0.12	0.16	0.25	0.45
NO_x (μM)	3.26	3.89	0.20	0.21	0.25	0.23	5.79	6.23	8.24
NH₄⁺ (μM)	0.13	0.11	0.13	0.12	0.14	0.15	0.09	0.08	0.03
Total N (μM)	3.68	4.36	0.40	0.41	0.48	0.46	6.33	6.90	8.89
N:P ratio	23.81	35.74	4.00	3.42	3.43	3.83	39.56	27.60	19.76
Sequencing statistics
Total bp (Gb)	15.9	15.3	19.9	15.1	3.1	15.3	16.9	15.0	14.8
Mean read length (bp)	92	91.9	121.4	117.2	112.3	119.8	121.4	120.2	117.0
Mean GC (%)	40.7	41.1	38.6	41.4	40.4	40.6	41.3	41.1	45.9
Assembly statistics
Total bp (Mb)	203	201	738.5	500.3	79.5	577.3	701.3	613.5	490.0
Mean GC (%)	38	39	38.5	35.7	35.5	38.6	38.9	40	42.2
Maximum contig length (Kb)	137	109	251	165	118	235	186	140	450
Contigs > 1 Kb	39,794	38,491	172,484	125,642	17,760	145,031	173,153	153,245	115,711
Contigs > 10 Kb	869	885	4648	2198	202	2360	2789	2556	1807

ISSN: 2049-2618