Fig. 3From: Disruption of maternal gut microbiota during gestation alters offspring microbiota and immunityMaternal oral vancomycin impacts spleen cellularity at 14 days postpartum. Pups born to vancomycin breeders were sacrificed 14 days postpartum and their spleen lymphocyte populations characterized by FACS. a Total spleen cell counts of Pc (brown), Pgn (blue), Pg (gray), and Pn (yellow). b Proportions and c numbers of CD3+ cells that are CD4+. d Representative flow plot of effector and central memory CD4+ T cell subsets in spleen of Pc versus Pgn, gated from CD4+ population. Frequency of CD3+CD4+ that are e CD44hiCD62Lhi (central memory CD4 T cells) and f CD44hiCD62Llo (effector memory CD4 T cells). Graphs shown as mean ± SEM. Data are representative of two independent experiments. n = 6–10 pups per group. *p < 0.05, **p < 0.01, ***p < 0.001. Kruskal-Wallis test followed by Mann-Whitney U testBack to article page