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Fig. 3 | Microbiome

Fig. 3

From: Disruption of maternal gut microbiota during gestation alters offspring microbiota and immunity

Fig. 3

Maternal oral vancomycin impacts spleen cellularity at 14 days postpartum. Pups born to vancomycin breeders were sacrificed 14 days postpartum and their spleen lymphocyte populations characterized by FACS. a Total spleen cell counts of Pc (brown), Pgn (blue), Pg (gray), and Pn (yellow). b Proportions and c numbers of CD3+ cells that are CD4+. d Representative flow plot of effector and central memory CD4+ T cell subsets in spleen of Pc versus Pgn, gated from CD4+ population. Frequency of CD3+CD4+ that are e CD44hiCD62Lhi (central memory CD4 T cells) and f CD44hiCD62Llo (effector memory CD4 T cells). Graphs shown as mean ± SEM. Data are representative of two independent experiments. n = 6–10 pups per group. *p < 0.05, **p < 0.01, ***p < 0.001. Kruskal-Wallis test followed by Mann-Whitney U test

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