Microbiome

Table 2 Bile acid and cholesterol concentrations in systemic plasma of fasted mice

From: The gut microbiota drives the impact of bile acids and fat source in diet on mouse metabolism

Colonization status	GF		SPF
Diet	PHB	LHB	PHB	LHB
T-α-MCA [nM]	107 ± 60	163 ± 128	31 ± 11	53 ± 31
T-β-MCA [nM]	358 ± 125 ^a	780 ± 617	36 ± 10 ^c	34 ± 17 ^d
TCA [nM]	186 ± 116	236 ± 173	39 ± 54	35 ± 23 ^d
TCDCA [nM]	23 ± 28	43 ± 51	7.1 ± 8.4	22 ± 13 ^b
TDCA [nM]	BQ	BQ	53 ± 19	83 ± 32
β-MCA [nM]	29 ± 26	134 ± 225	31 ± 40	21 ± 37
12-DHCA [nM]	BQ	BQ	2.2 ± 4.0	1.6 ± 3.8
CA [nM]	3.8 ± 6.5	7.6 ± 10.8	45 ± 39	37 ± 25
TLCA [nM]	BQ	BQ	BQ	BQ
DCA [nM]	BQ	BQ	103 ± 32	142 ± 55
SUM of all bile acids [nM]	706 ± 348	1363 ± 1179	347 ± 95	429 ± 151 ^d
Primary [nM]	706 ± 348	1363 ± 1179	189 ± 87	202 ± 103 ^d
Secondary [nM]	BQ	BQ	159 ± 48	227 ± 85
Tauro-conjugated [nM]	673 ± 326	1221 ± 963	166 ± 59	228 ± 61 ^d
Unconjugated [nM]	33 ± 28	142 ± 220	181 ± 83	201 ± 104
Cholesterol [μM]	628 ± 241	539 ± 234	775 ± 242	790 ± 235

Data are mean ± SD. Diets are as in Table 1. Italicized data indicate differences between groups. Superscript letters indicate statistical significance (p < 0.05, n = 4–6; two-way ANOVA with Holm-Sidak for multiple comparison; t test for LHB vs. PHB comparisons within colonization groups) as follows: ^aP- vs. LHB among GF mice; ^bP- vs. LHB among SPF mice; ^cGF vs. SPF for PHB diet; ^dGF vs. SPF for LHB diet. CA cholic acid, CDCA chenodeoxycholic acid, DCA deoxycholic acid, DHCA dihydroxycholic acid, LCA lithocholic acid, MCA muricholic acid, T tauro-conjugated, BQ below quantification limit [38]

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