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Table 2 Bile acid and cholesterol concentrations in systemic plasma of fasted mice

From: The gut microbiota drives the impact of bile acids and fat source in diet on mouse metabolism

Colonization status GF SPF
Diet PHB LHB PHB LHB
T-α-MCA [nM] 107 ± 60 163 ± 128 31 ± 11 53 ± 31
T-β-MCA [nM] 358 ± 125 a 780 ± 617 36 ± 10 c 34 ± 17 d
TCA [nM] 186 ± 116 236 ± 173 39 ± 54 35 ± 23 d
TCDCA [nM] 23 ± 28 43 ± 51 7.1 ± 8.4 22 ± 13 b
TDCA [nM] BQ BQ 53 ± 19 83 ± 32
β-MCA [nM] 29 ± 26 134 ± 225 31 ± 40 21 ± 37
12-DHCA [nM] BQ BQ 2.2 ± 4.0 1.6 ± 3.8
CA [nM] 3.8 ± 6.5 7.6 ± 10.8 45 ± 39 37 ± 25
TLCA [nM] BQ BQ BQ BQ
DCA [nM] BQ BQ 103 ± 32 142 ± 55
SUM of all bile acids [nM] 706 ± 348 1363 ± 1179 347 ± 95 429 ± 151 d
 Primary [nM] 706 ± 348 1363 ± 1179 189 ± 87 202 ± 103 d
 Secondary [nM] BQ BQ 159 ± 48 227 ± 85
 Tauro-conjugated [nM] 673 ± 326 1221 ± 963 166 ± 59 228 ± 61 d
 Unconjugated [nM] 33 ± 28 142 ± 220 181 ± 83 201 ± 104
Cholesterol [μM] 628 ± 241 539 ± 234 775 ± 242 790 ± 235
  1. Data are mean ± SD. Diets are as in Table 1. Italicized data indicate differences between groups. Superscript letters indicate statistical significance (p < 0.05, n = 4–6; two-way ANOVA with Holm-Sidak for multiple comparison; t test for LHB vs. PHB comparisons within colonization groups) as follows: aP- vs. LHB among GF mice; bP- vs. LHB among SPF mice; cGF vs. SPF for PHB diet; dGF vs. SPF for LHB diet. CA cholic acid, CDCA chenodeoxycholic acid, DCA deoxycholic acid, DHCA dihydroxycholic acid, LCA lithocholic acid, MCA muricholic acid, T tauro-conjugated, BQ below quantification limit [38]