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Fig. 1 | Microbiome

Fig. 1

From: The gut microbiota drives the impact of bile acids and fat source in diet on mouse metabolism

Fig. 1

Impact of experimental feedings and microbial colonization on mouse metabolism. a Final body weight (at the age of 18 weeks after 8 weeks of feeding). b WAT mass for all groups and representative pictures of respective fat depots collected from SPF mice fed the palm oil- or lard-based diets. c Corresponding regression analysis of WAT mass and body weight. d Blood glucose concentrations during OGTT with corresponding areas under the curve. Color code for diets: CD, black; BA, red; PHB, dark blue; LHB, cyan blue. Symbols for colonization status: GF, diamonds; SPF, filled triangles. All mice used in the experiments are shown (group size varied as indicated below the x-axis). See the “Methods” section for description of statistical analyses. e Quantification of glucagon-like peptide (GLP) 1-positive cells in colonic tissue sections of SPF mice from the different feeding groups. At least three non-consecutive sections were stained from each mouse and quantified. Symbols represent average values from individual mice. Representative pictures of immunohistochemical staining acquired with a confocal microscope are shown (for the sake of space and appropriate size of images, picture for CD and BA are not shown but are equivalent to LHB group). Arrows indicate cells positive for GLP1. The black bars indicate 100 μm. ***p < 0.01, one-way ANOVA followed by the Tukey test (performed using Graph Pad Prism)

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