Fig. 1

Effects of virus enrichment and random amplification on mock viral communities. Relative proportions of seven DNA viruses (lambda = bacteriophage lambda; WR = vaccinia WR; phi29 = bacteriophage φ29; AdenoV = human adenovirus 5; M13 = bacteriophage M13; MVMp = Minute Virus of Mice p; PCV2a = porcine circovirus 2a) from mock community 1 (a) and mock community 2 (b) were assessed by qPCR before and after single or combined treatments. Two or three independent replicates were tested for each experiment and noted with numbers 1–3. Sample names include the following identifiers related to treatment: Control, untreated mock assemblage; C, two consecutive centrifugation steps at 3000×g for 10 min; 0.45 and 0.22, filter pore size (μm) used during syringe filtration; I, iodixanol cushion; MDA, multiple displacement amplification with GenomiPhi kit; MDA_WD, multiple displacement amplification without denaturation step; SISPA, sequence-independent, single-primer amplification. Fold change in the total amount of each virus genome before and after a given treatment is shown for mock community 1 (c) and 2 (d). Fold change in relative viral proportion before and after random amplification treatments is shown for mock community 1 (e) and 2 (f)