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Table 2 Effect of spike level on estimated copy number of rRNA genes

From: Absolute quantitation of microbiota abundance in environmental samples

Fraction

Bawburgh

Wytham

 

Mean

SEM

Mean

SEM

16S rRNA all

1.9E + 09

2.5E + 08

4.1E + 09

7.2E + 08

16S rRNA 200–800

1.6E + 09

2.3E + 08

2.2E + 09

2.3E + 08

18S rRNA all

1.5E + 08

2.4E + 07

2.2E + 08

3.6E + 07

18S rRNA 200–800

1.5E + 08

5.6E + 06

2.1E + 08

1.4E + 07

ITS all

5.6E + 06

9.8E + 05

7.7E + 06

1.7E + 06

ITS 200–800

8.3E + 06

8.9E + 05

1.4E + 07

1.7E + 06

  1. Copies of the target gene are shown calculated from samples with addition of synthetic spikes at levels 1–8 (Table 1) (all) and for those which fulfil the criteria > 200 and < 800 synthetic spike reads per 1000 total reads (200–800). For 16S rRNA, gene 200–800 zone is levels 7 and 8, for 18S rRNA is levels 3,4, 5 and 6 and for ITS is levels 1 and 2
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Microbiome

ISSN: 2049-2618

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