Fig. 4

Genus level distribution of fecal microbiota between offspring of fat-1 and WT mothers. a Least discriminant analysis of effect size (LEfSe) on clustered groups based on maternal genotype revealed that Firmicutes were more abundant in offspring of WT foster mothers whereas Bacteroidetes and Proteobacteria were more abundant in offspring of fat-1 foster mothers. b Normalized data of fecal microbiota relative abundance revealed distinct differences between offspring groups. Microbiota composition appeared similar in groups of the same foster mother genotype. Data represents OTUs with significantly different relevant abundances between treatment groups (as determined by Kruskall-Wallis testing and Benjamani-Hocherg multiple correction testing). Each row represents an OTU labeled by lowest taxonomic description and OTU ID, normalized to the row maximum. Data normalized per taxonomic read. c Clostridia displayed significantly higher relative abundance in offspring of WT foster mothers. Bacteroides displayed higher relative abundance in offspring of fat-1 foster mothers. Akkermansia displayed the highest relative abundance in fat-1/fat-1 offspring. Epsilonproteobacteria were almost entirely depleted (< 0.1%) in offspring fostered to WT mothers. n = 5–10 per group, n = 1–4 per cage. Significant differences were determined by non-parametric analysis using the Kruskall-Wallis test followed by Mann-Whitney test and FDR correction by Benjamani-Hochburg testing. Groups with different letters are significantly different