Fig. 3

Phylum compositional and diversity differences in fecal microbiota between offspring of fat-1 and WT mothers. a Analysis of beta diversity identified significant clustering of offspring groups by foster maternal genotype. b α-diversity as measured by the Shannon index did not differ between post-HFD groups, however, appeared to be reduced in WT/WT compared with WT/fat-1 before HFD feeding. c The Firmicutes:Bacteroidetes ratio was lowest in fat-1/fat-1 offspring. d Fecal microbiota composition differed significantly between offspring groups at phylum level and appeared dependent on foster mother genotype. Significant differences were determined by non-parametric analysis using the Kruskall-Wallis test followed by Mann-Whitney test. n = 5–10 per group, n = 1–4 per cage. Groups with different letters are significantly different