Near full-length 16S rRNA gene next-generation sequencing revealed Asaia as a common midgut bacterium of wild and domesticated Queensland fruit fly larvae

Table 1 Bactrocera tryoni larval populations used in this study

Suffix used in this study	Wild or domesticated (generation)	Substrate	Source	Sampling date^d	Number of larvae analyzed by NGS
Bux.P	Wild	Peach	Residential property, Buxton, NSW	Jan 2015	20 (from 7 fruit)
Tum.P	Wild	Peach	Residential property, Tumut, NSW	Feb 2015	15 (from 5 fruit)
GPII.Col^a	Domesticated (F24)	Artificial carrot diet	Gosford Primary Industries Institute, Ourimbah, NSW	Dec 2014	9
FFPF.Col^a,b	Domesticated (F78-81)	Artificial carrot diet	Fruit Fly Production Facility, Menangle, NSW	Dec 2015	8
MQ.Col^a	Domesticated (unknown)^c	Artificial carrot diet	Macquarie University, North Ryde, NSW	June 2015	6

^aAll of these colonies were derived from the same source colony but were maintained at different locations
^bThis colony was reared on lucerne chaff larval diet for at least 40 generations
^cThe generation of this colony is unknown, except it is greater than 24 generations
^dSampling date for both domesticated and wild larvae

ISSN: 2049-2618