Fig. 4
From: A comparison of methods used to unveil the genetic and metabolic pool in the built environment
Differences in the DNA and RNA components of the indoor air microbiome. a Distance-based redundancy analysis of community composition as measured by weighted UniFrac distances between DNA (blue) and RNA (red) components of the microbiome. Top genera are indicated in gray fonts, and their potential roles in driving the different microbiome components are represented by linear lines. b Taxonomic profiles of DNA and RNA components of the microbiome. The top 15 genera based on overall relative abundance across the dataset are presented, with the remaining genera and those without genus-level taxonomic classification grouped as “minor/unclassified.” c DeSeq2 analysis indicating the OTUs significantly enriched (i.e., FDR-corrected p < 0.05) in the DNA (blue) and RNA (red) components of the indoor air microbiome. Genus-level classification are provided for each OTU where available. All analyses showed that the genetic pool (DNA, blue) was strongly characterized by environmental genera that were less likely to be metabolically active, whereas host-associated genera characterized the viable population (RNA)