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Fig. 2 | Microbiome

Fig. 2

From: A comparison of methods used to unveil the genetic and metabolic pool in the built environment

Fig. 2

In vitro DNA/RNA co-extraction efficiencies tests. a Co-extraction protocol comparison. FastPrep: co-extraction protocol using the FastDNA and FastRNA SPIN Kit for Yeast; AllPrep: using the AllPrep DNA/RNA Mini Kit; Power: using DNeasy PowerSoil Kit and PowerMicrobiome RNA Isolation Kit. FastPrep method was the most efficient co-extraction method. b Comparison of the recovery efficiency from different type of surfaces (plastic, metal, and untreated wood trays) using different swabs (eSwab, BBL Culture Swab, and BiSKit) and the FastPrep co-extraction protocol. No significant differences were detected between swabs or sampled surfaces. The fungi had typically higher recovery rates than bacteria while P. syringae consistently had the lowest recovery for both nucleic acids

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