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Fig. 4 | Microbiome

Fig. 4

From: Reproducible protocols for metagenomic analysis of human faecal phageomes

Fig. 4

Inter-operator bias in faecal phageome and bacteriome profiling. a Outline of experimental protocol: fresh samples were collected from 6 individuals (3 healthy and 3 IBD patients) and processed twice each for phageome and total faecal DNA by each of the three laboratory technicians. b Within- and between-subject Spearman distances of relative abundance values per sample in datasets obtained using metagenomic sequencing of phageomes and 16S rRNA amplicon sequencing. c, d PCoA plots based on Spearman distances calculated for 16S rRNA amplicon and phageome datasets, respectively. e, f Heatplots of relative abundances of selected bacterial RSVs and viral contigs (> 0.5% in any of the samples) across samples collected in the experiment

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