Fig. 2

Effect of different concentrations of OTC on Daphnia microbiota after 23 days of exposure. In all graphs, individual populations are indicated with the same combination of color (OTC concentration) and shape (different populations within a specific OTC concentration). A–C Bacterial load, OTU richness, and Shannon diversity. Bars indicate mean values for each OTC concentration; points indicate specific values for one population. B, C Letters above bars indicate significant differences between OTC concentrations at 5% as determined by a Tukey HSD post hoc test. D Relative abundance of OTUs belonging to the Proteobacteria; color indicates proteobacterial class and letters inside the bars indicate specific OTUs (A = Acinetobacter sp., H = Hydrogenophaga sp., N = Neisseriaceae sp.). E PCoA of Daphnia microbiota in different populations using weighted Unifrac distances