Fig. 1

High salt diet alters gut microbiota composition and function. a Principal coordinate analysis (PCoA) plots of unweighted UniFrac metric (left panel) and abundance Jaccard index (right panel) of fecal microbiota composition over time exposed to HSD. Gray arrows indicate the direction of the significant microbiota composition that shifts between groups. b Summary of the relative abundance of microbial genera present in 99.9% of the community overtime exposure to HSD. c Relative abundance of fecal bacterial genera (left panel) and OTUs (right panel) that significantly correlate with HSD (q < 0.05). The blue background represents the period exposed to HSD. d Heatmap of the 20 most significant KEGG pathways that correlate with HSD in fecal microbiota. e SCFA quantification at 0 and 4 weeks after HSD. Un. G (Unclassified Genus). AA (acetic acid), PrA (propionic acid), IbuA (isobutyric acid), BA (butyric acid), IvaA (isovaleric acid), PeA (pentanoic acid), LA (lactic acid). Values are presented as means ± SEM. n = 5–6 mice/group. *p < 0.05