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Table 2 Summary of weighted UniFrac and the PERMANOVA test statistics

From: Holobionts and ecological speciation: the intestinal microbiota of lake whitefish species pairs

Comparison Lakes/effects UniFrac PERMANOVA
WSig F value R2 Pr (> F)
Both whitefish species microbiota-water bacterial communities (sequence dataset) Water-whitefish < 0.0010 33.834 0.185 < 0.0010
Lakes 2.774 0.061 < 0.0010
Water-whitefish*lake 1.278 0.028 0.074
Cliff < 0.0010 3.818 0.124 < 0.0010
East < 0.0010 6.910 0.210 < 0.0010
Indian < 0.0010 6.653 0.172 < 0.0010
Témiscouata < 0.0010 6.218 0.182 < 0.0010
Webster < 0.0010 7.341 0.269 < 0.0010
Dwarf-normal whitefish microbiota (sequence dataset) Species < 0.0010 2.273 0.019 0.002
Lakes 2.812 0.096 < 0.0010
Species*lake 1.493 0.051 0.0021
Cliff < 0.0010 1.931 0.081 0.006
East < 0.0010 1.821 0.092 0.019
Indian < 0.0010 0.913 0.037 0.530
Témiscouata < 0.0010 1.848 0.077 0.025
Webster < 0.0010 1.396 0.104 0.145
Dwarf-normal whitefish microbiota (PICRUSt results) Species 0.448 0.003 0.697
Lake 6.761 0.200 < 0.0010
Species*lake 2. 273 0.067 0.016
Cliff 0.152 0.007 0.958
East 1.642 0.083 0.114
Indian 0.413 0.017 0.793
Témiscouata 5.052 0.186 0.019
Webster 2.562 0.176 0.108
  1. Three comparisons are shown: (i) comparison within and between lakes for the whitefish microbiota (dwarf and normal) and the water bacterial communities, (ii) comparison within and between lakes for the dwarf whitefish microbiota and the normal whitefish microbiota, and (iii) comparison of the PICRUSt results between dwarf and normal microbiota for all lakes combined and for each lake. For the three comparisons, we tested the lake effect and the interaction between the bacterial communities (water, dwarf, and normal whitefish) using PERMANOVA. UniFrac test is based on beta diversity and cannot be done with PICRUSt results