Integrated multi-omic analysis of host-microbiota interactions in acute oak decline

Microbiome

Table 1 List of genes and proteins of interest involved in virulence, bacterial survival and interactions identified in the metaproteome and metatranscriptome of AOD lesions and their corresponding bacterial species/group of origin

Gene annotation	B. goodwinii	G. quercinecans	R. victoriana	G+	G−
Antitoxin HicB	T
Bacterial type II secretion system protein F domain protein	T
Protease CtpB				T
Catalase	PT	T		T	T
Colicin V secretion protein CvaA	T	P
CRISPR-associated protein Csy3		T
CsrB	T	T	T		T
Cysteine protease avirulence protein AvrPphB	T
Effector protein HopM1	T
Effector protein YopJ	T
Entericidin B membrane lipoprotein	P
Flavodoxin	PT
Harpin HrpN	T
HTH-type transcriptional regulator KdgR	T
Iron-binding protein IscA	T
Multidrug efflux pump subunit AcrB	T		T		T
Oligoendopeptidase F, plasmid				PT
Oligogalacturonate lyase	T
Oligopeptidase A	T
Pathogenicity factor	T
Pectate lyase A precursor	T
Peroxiredoxin OsmC	PT
Persistence and stress-resistance toxin PasT	T
ProP effector	T
Protease 2	T
Protease HtpX	T
Putative oxidoreductase SadH		T
Putative type II secretion system protein E	T			T
Response regulator UvrY	T
Serine protease AprX				T
Superoxide dismutase [Mn]	PT			T
Thermostable beta-glucosidase B	T			T
Toxin A				PT
Toxin B				T
Toxin HigB-2	T
Toxin-antitoxin biofilm protein TabA	T
Type II secretion system protein D	T				PT
Type IV secretion system protein VirB		T
Virulence factor SrfB	T
Virulence regulon transcriptional activator VirF	T
Virulence sensor histidine kinase PhoQ	T

G+ signifies the two Gram-positive bacteria belonging to the Clostridioides and Carnobacterium, while G− signifies other Gram-negative bacteria excluding B. goodwinii, G. quercinecans and R. victoriana. P signifies detection of that gene in the proteomic data, while T signifies detection of the gene in the transcriptomic data from AOD lesions

ISSN: 2049-2618