Effect of heat shock on hot water plumbing microbiota and Legionella pneumophila control

Microbiome

Table 1 Statistical comparison of samples collected pre- and post-heat shock (permutation = 999)

	Weighted UniFrac			Unweighted UniFrac
	R ² _Adonis	P _Adonis	P _Betadisp	R ² _Adonis	P _Adonis	P _Betadisp
a. “Heat shock” rig, biofilm samples
2-month pre- (19) vs 2-month post- (19)	0.0760	0.020	0.919	0.0737	0.001	0.430
2-month pre- (19) vs immediately pre- (18)	0.0310	0.307	0.257	0.0352	0.098	0.901
Immediately pre- (18) vs 2-month post- (19)	0.0623	0.056	0.297	0.0553	0.001	0.416
b. “Thermal disruption” rig, biofilm samples
2-month pre- (19) vs 2-month post- (19)	0.2104	0.001	0.169	0.1525	0.001	0.001
2-month pre- (19) vs immediately pre- (18)	0.0202	0.566	0.781	0.0292	0.299	0.995
Immediately pre- (18) vs 2-month post- (19)	0.2193	0.001	0.128	0.1504	0.001	0.001
c. “Heat shock” rig, water samples
2-month pre- (20) vs 2-month post- (21)	0.1434	0.003	0.018	0.0789	0.001	0.019
2-month pre- (20) vs immediately pre- (18)	0.2274	0.001	0.400	0.0598	0.001	0.604
Immediately pre- (18) vs 2-month post- (21)	0.2209	0.001	0.006	0.0763	0.001	0.005
d. “Thermal disruption” rig, water samples
2-month pre- (20) vs 2-month post- (21)	0.3808	0.001	0.340	0.1737	0.001	0.499
2-month pre- (20) vs immediately pre- (18)	0.1038	0.009	0.471	0.0455	0.003	0.478
Immediately pre- (18) vs 2-month post- (21)	0.2498	0.001	0.056	0.1593	0.001	0.979

ISSN: 2049-2618