Table 1 The set of six primer pairs used in this study. The number of Greengenes sequences (out of the total 1,402,801 sequences) that have a perfect match with each primer pair. The primers’ sequences and their median start locations along the forward strand are shown. The set of primers was used for profiling our mock mixture, the D. melanogaster samples, and for in silico simulations
From: Combining 16S rRNA gene variable regions enables high-resolution microbial community profiling
Region | # amplified | Forward primer | Reverse primer | ||
|---|---|---|---|---|---|
Sequence | Position | Sequence | Position | ||
1 | 196415 | 5′-TGGCGGACGGGTGAGTAA-3′ | 74 | 5′- CTGCTGCCTCCCGTAGGA-3′ | 315 |
2 | 1122118 | 5′-TCCTACGGGAGGCAGCAG-3′ | 316 | 5′- TATTACCGCGGCTGCTGG-3′ | 484 |
3 | 660912 | 5′-CAGCAGCCGCGGTAATAC-3′ | 486 | 5′- CGCATTTCACCGCTACAC-3′ | 650 |
4 | 660342 | 5′-AGGATTAGATACCCTGGT-3′ | 752 | 5′- GAATTAAACCACATGCTC-3′ | 911 |
5 | 591604 | 5′-GCACAAGCGGTGGAGCAT-3′ | 901 | 5′- CGCTCGTTGCGGGACTTA-3′ | 1057 |
6 | 783882 | 5′-AGGAAGGTGGGGATGACG-3′ | 1143 | 5′- CCCGGGAACGTATTCACC-3′ | 1336 |