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Table 3 Organisms and their recommended growing conditions. Each of the three microbes used in co-culture validation experiments was purchased from ATCC, rehydrated, and grown under their recommended conditions before co-culturing began. The ATCC catalogue numbers and recommended growing conditions are presented here. Because P. acnes is an aerotolerant anaerobe, it was first grown in a homemade anaerobic jar inside the incubator

From: Fungi stabilize connectivity in the lung and skin microbial ecosystems

Organism ATCC catalogue number Recommended temperature Recommended media
Emericella nidulans 96,921 24 °C Malt extract agar
Propionibacterium acnes 6919 37 °C Tryptic soy agar with 5% sheep blood
Rothia dentocariosa 17,931 37 °C Brain-heart infusion agar