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Fig. 1 | Microbiome

Fig. 1

From: Quinones are growth factors for the human gut microbiota

Fig. 1

Isolating uncultured bacteria using co-culture methods. Two approaches are used to identify bacterial isolates from stool exhibiting dependency phenotypes. a In an untargeted approach, early-forming colonies are picked and spotted on plates where late-forming neighboring colonies are spread. b In a targeted approach, a known helper bacterium, in this case Escherichia coli K12, can be spotted at the time of inoculation of stool sample. Neighboring colonies around the E. coli spot are then picked, spread on fresh agar, and an E. coli inoculum is then spotted on the plate. In both assays, induction of the candidate-dependent bacteria around the spotted helper indicates a positive hit. The plates in this figure are used as a representative example to explain the isolation technique

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