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Fig. 1 | Microbiome

Fig. 1

From: Towards a balanced view of the bacterial tree of life

Fig. 1

Bacterial SSU rRNA gene-based maximum-likelihood phylogenetic tree. Shown are representative taxa based on all SSU rRNA genes ≥ 1200 bp extracted from 6744 metagenomes deposited in IMG/M [10] and reference genomes (isolates, single amplified genomes, and metagenome-assembled genomes), which were consecutively de-replicated at 97% (97% OTUs) and then clustered at 85% sequence similarity thresholds (85% clusters). Bacterial phyla represented by at least two 85% clusters are shown, and for each phylum, bootstrap support is indicated if greater than 50. Branches in the tree are colored in turquoise if clusters contain SSU rRNA genes extracted from genome sequences. Sizes of pie charts correlate with the total number of 97% OTUs in the respective phylum (log2 transformed) and are divided based on percentage of 97% OTUs assigned to any of the three categories: (1) OTUs consisting of metagenomic SSU rRNA sequences with at least one member found in the SILVA database (MG & SILVA, yellow), (2) OTUs consisting solely of metagenomic sequences derived from this study (MG only, red), and (3) OTUs containing SSU rRNA genes extracted from reference genomes. Eighty-five percent clusters marked with filled black circles would likely have been missed by amplicon studies using the Earth Microbiome primer set (515f/806r) [14], with the size of the filled circles correlating with the mismatch score (indicated are mismatch scores greater than 1 [4]). The tree was rooted based on a representative set of 15 archaeal SSU rRNA gene sequences

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