TY - JOUR AU - Petigura, E. A. AU - Howard, A. W. AU - Marcy, G. W. PY - 2013 DA - 2013// TI - Prevalence of Earth-size planets orbiting sun-like stars JO - Proc Natl Acad Sci National Acad Sciences VL - 110 UR - https://doi.org/10.1073/pnas.1319909110 DO - 10.1073/pnas.1319909110 ID - Petigura2013 ER - TY - JOUR AU - Rettberg, P. AU - Anesio, A. M. AU - Baker, V. R. AU - Baross, J. A. AU - Cady, S. L. AU - Detsis, E. PY - 2016 DA - 2016// TI - Planetary protection and Mars special regions—a suggestion for updating the definition JO - Astrobiology VL - 16 UR - https://doi.org/10.1089/ast.2016.1472 DO - 10.1089/ast.2016.1472 ID - Rettberg2016 ER - TY - STD TI - Kminek G, Rummel JD. COSPAR’s planetary protection policy. Sp Res Today. 2015; Available: https://cosparhq.cnes.fr/sites/default/files/ppp_article_linked_to_ppp_webpage.pdf UR - https://cosparhq.cnes.fr/sites/default/files/ppp_article_linked_to_ppp_webpage.pdf ID - ref3 ER - TY - JOUR AU - Formisano, V. AU - Atreya, S. AU - Encrenaz, T. AU - Ignatiev, N. AU - Giuranna, M. PY - 2004 DA - 2004// TI - Detection of methane in the atmosphere of Mars JO - Science VL - 306 UR - https://doi.org/10.1126/science.1101732 DO - 10.1126/science.1101732 ID - Formisano2004 ER - TY - JOUR AU - Webster, C. R. AU - Mahaffy, P. R. AU - Atreya, S. K. AU - Flesch, G. J. AU - Mischna, M. A. AU - Meslin, P. -. Y. PY - 2015 DA - 2015// TI - Mars methane detection and variability at gale crater JO - Science (80-) VL - 347 UR - https://doi.org/10.1126/science.1261713 DO - 10.1126/science.1261713 ID - Webster2015 ER - TY - JOUR AU - Kirschke, S. AU - Bousquet, P. AU - Ciais, P. AU - Saunois, M. AU - Canadell, J. G. AU - Dlugokencky, E. J. PY - 2013 DA - 2013// TI - Three decades of global methane sources and sinks JO - Nat Geosci VL - 6 UR - https://doi.org/10.1038/ngeo1955 DO - 10.1038/ngeo1955 ID - Kirschke2013 ER - TY - STD TI - Sender R, Fuchs S, Milo R. Revised estimates for the number of human and bacteria cells in the body. bioRxiv. 2016; doi:10.1101/036103. ID - ref7 ER - TY - JOUR AU - Meadow, J. F. AU - Altrichter, A. E. AU - Bateman, A. C. AU - Stenson, J. AU - Brown, G. Z. AU - Green, J. L. PY - 2015 DA - 2015// TI - Humans differ in their personal microbial cloud JO - PeerJ VL - 3 UR - https://doi.org/10.7717/peerj.1258 DO - 10.7717/peerj.1258 ID - Meadow2015 ER - TY - JOUR AU - Mora, M. AU - Mahnert, A. AU - Koskinen, K. AU - Pausan, M. R. AU - Oberauner-Wappis, L. AU - Krause, R. PY - 2016 DA - 2016// TI - Microorganisms in confined habitats: microbial monitoring and control of the International Space Station, cleanrooms, operating rooms and intensive care units JO - Front Microbiol VL - 7 UR - https://doi.org/10.3389/fmicb.2016.01573 DO - 10.3389/fmicb.2016.01573 ID - Mora2016 ER - TY - STD TI - Mahnert A, Moissl-Eichinger C, Berg G, Vaishampayan PA, Probst AJ, Auerbach AK, et al. Molecular bacterial community analysis of clean rooms where spacecraft are assembled. Moldenhauer J, editor. Astrobiology. 2016/02/03 ed. Pasadena, CA: Elsevier; 2015;3: 1. doi:10.1126/science.aad1329. ID - ref10 ER - TY - STD TI - Moissl-Eichinger C, Cockell C, Rettberg P. Venturing into new realms? Microorganisms in space. FEMS Rev. 2016; doi:10.1093/femsre/fuw015. ID - ref11 ER - TY - JOUR AU - Puleo, J. R. AU - Fields, N. D. AU - Bergstrom, S. L. AU - Oxborrow, G. S. AU - Stabekis, P. D. AU - Koukol, R. PY - 1977 DA - 1977// TI - Microbiological profiles of the Viking spacecraft JO - Appl Environ Microbiol VL - 33 ID - Puleo1977 ER - TY - STD TI - Microbial examination of flight hardware and cleanrooms ECSS-Q-ST-70-55C. 2008. ID - ref13 ER - TY - STD TI - Administration NA and S. Handbook for the microbial examination of space hardware: NASA Tech Handb; 2010. ID - ref14 ER - TY - STD TI - Vaishampayan P, Osman S, Andersen G, Venkateswaran K. High-density 16S microarray and clone library-based microbial community composition of the Phoenix spacecraft assembly clean room. Astrobiology. 2010;10 doi:10.1089/ast.2009.0443. ID - ref15 ER - TY - JOUR AU - Mahnert, A. AU - Vaishampayan, P. AU - Probst, A. J. AU - Auerbach, A. AU - Moissl-Eichinger, C. AU - Venkateswaran, K. PY - 2015 DA - 2015// TI - Cleanroom maintenance significantly reduces abundance but not diversity of indoor microbiomes JO - PLoS One VL - 10 UR - https://doi.org/10.1371/journal.pone.0134848 DO - 10.1371/journal.pone.0134848 ID - Mahnert2015 ER - TY - JOUR AU - Vaishampayan, P. AU - Probst, A. J. AU - La Duc, M. T. AU - Bargoma, E. AU - Benardini, J. N. AU - Andersen, G. L. PY - 2013 DA - 2013// TI - New perspectives on viable microbial communities in low-biomass cleanroom environments JO - ISME J. VL - 7 UR - https://doi.org/10.1038/ismej.2012.114 DO - 10.1038/ismej.2012.114 ID - Vaishampayan2013 ER - TY - JOUR AU - Moissl-Eichinger, C. AU - Auerbach, A. K. AU - Probst, A. J. AU - Mahnert, A. AU - Tom, L. AU - Piceno, Y. PY - 2015 DA - 2015// TI - Quo vadis? Microbial profiling revealed strong effects of cleanroom maintenance and routes of contamination in indoor environments JO - Sci Rep VL - 5 UR - https://doi.org/10.1038/srep09156 DO - 10.1038/srep09156 ID - Moissl-Eichinger2015 ER - TY - JOUR AU - Probst, A. AU - Facius, R. AU - Wirth, R. AU - Moissl-Eichinger, C. PY - 2010 DA - 2010// TI - Validation of a nylon-flocked-swab protocol for efficient recovery of bacterial spores from smooth and rough surfaces JO - Appl Environ Microbiol VL - 76 UR - https://doi.org/10.1128/AEM.00399-10 DO - 10.1128/AEM.00399-10 ID - Probst2010 ER - TY - JOUR AU - Vaishampayan, P. AU - Probst, A. AU - Krishnamurthi, S. AU - Ghosh, S. AU - Osman, S. AU - McDowall, A. PY - 2010 DA - 2010// TI - Bacillus horneckiae sp. nov., isolated from a spacecraft-assembly clean room JO - Int J Syst Evol Microbiol VL - 60 UR - https://doi.org/10.1099/ijs.0.008979-0 DO - 10.1099/ijs.0.008979-0 ID - Vaishampayan2010 ER - TY - JOUR AU - Behrendt, U. AU - Schumann, P. AU - Stieglmeier, M. AU - Pukall, R. AU - Augustin, J. AU - Spröer, C. PY - 2010 DA - 2010// TI - Characterization of heterotrophic nitrifying bacteria with respiratory ammonification and denitrification activity–description of Paenibacillus uliginis sp. nov., an inhabitant of fen peat soil and Paenibacillus purispatii sp. nov., isolated from a spacec JO - Syst Appl Microbiol VL - 33 UR - https://doi.org/10.1016/j.syapm.2010.07.004 DO - 10.1016/j.syapm.2010.07.004 ID - Behrendt2010 ER - TY - JOUR AU - Weinmaier, T. AU - Probst, A. J. AU - Duc, M. T. AU - Ciobanu, D. AU - Cheng, J. -. F. AU - Ivanova, N. PY - 2015 DA - 2015// TI - A viability-linked metagenomic analysis of cleanroom environments: eukarya, prokaryotes, and viruses JO - Microbiome VL - 3 UR - https://doi.org/10.1186/s40168-015-0129-y DO - 10.1186/s40168-015-0129-y ID - Weinmaier2015 ER - TY - JOUR AU - Moissl-Eichinger, C. AU - Pukall, R. AU - Probst, A. J. AU - Stieglmeier, M. AU - Schwendner, P. AU - Mora, M. PY - 2013 DA - 2013// TI - Lessons learned from the microbial analysis of the Herschel spacecraft during assembly, integration, and test operations JO - Astrobiology VL - 13 UR - https://doi.org/10.1089/ast.2013.1024 DO - 10.1089/ast.2013.1024 ID - Moissl-Eichinger2013 ER - TY - JOUR AU - Moissl-Eichinger, C. PY - 2011 DA - 2011// TI - Archaea in artificial environments: their presence in global spacecraft clean rooms and impact on planetary protection JO - ISME J. VL - 5 UR - https://doi.org/10.1038/ismej.2010.124 DO - 10.1038/ismej.2010.124 ID - Moissl-Eichinger2011 ER - TY - JOUR AU - Moissl-Eichinger, C. AU - Rettberg, P. AU - Pukall, R. PY - 2012 DA - 2012// TI - The first collection of spacecraft-associated microorganisms: a public source for extremotolerant microorganisms from spacecraft assembly clean rooms JO - Astrobiology VL - 12 UR - https://doi.org/10.1089/ast.2012.0906 DO - 10.1089/ast.2012.0906 ID - Moissl-Eichinger2012 ER - TY - JOUR AU - Venkateswaran, K. AU - Vaishampayan, P. AU - Benardini Iii, J. N. AU - Rooney, A. P. AU - Spry, J. A. PY - 2014 DA - 2014// TI - Deposition of extreme-tolerant bacterial strains isolated during different phases of Phoenix spacecraft assembly in a public culture collection JO - Astrobiology VL - 14 UR - https://doi.org/10.1089/ast.2013.0978 DO - 10.1089/ast.2013.0978 ID - Venkateswaran2014 ER - TY - STD TI - Watve M, Shejval V, Sonawane C, Rahalkar M, Matapurkar A, Shouche Y, Patole M, Phadnis N, Champhenkar A, Damle K, Karandikar S, Kshirsagar V, Jog M. The “K” selected oligophilic bacteria: a key to uncultured diversity? Curr Sci. 2000;78:1535–42. ID - ref27 ER - TY - JOUR AU - Schumann, P. AU - Maier, T. PY - 2014 DA - 2014// TI - MALDI-TOF mass spectrometry applied to classification and identification of bacteria JO - Methods Microbiol Elsevier VL - 41 UR - https://doi.org/10.1016/bs.mim.2014.06.002 DO - 10.1016/bs.mim.2014.06.002 ID - Schumann2014 ER - TY - JOUR AU - Burggraf, S. AU - Olsen, G. J. AU - Stetter, K. O. AU - Woese, C. R. PY - 1992 DA - 1992// TI - A phylogenetic analysis of Aquifex pyrophilus JO - Syst Appl Microbiol Elsevier VL - 15 UR - https://doi.org/10.1016/S0723-2020(11)80207-9 DO - 10.1016/S0723-2020(11)80207-9 ID - Burggraf1992 ER - TY - CHAP AU - Lane, D. ED - Stackebrandt, E. ED - Goodfellow, M. PY - 1991 DA - 1991// TI - 16S/23S rRNA sequencing BT - Nucleic acid techniques in bacterial systematics PB - Wiley CY - Chichester ID - Lane1991 ER - TY - JOUR AU - Caporaso, J. G. AU - Lauber, C. L. AU - Walters, W. A. AU - Berg-Lyons, D. AU - Huntley, J. AU - Fierer, N. PY - 2012 DA - 2012// TI - Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms JO - ISME J. VL - 6 UR - https://doi.org/10.1038/ismej.2012.8 DO - 10.1038/ismej.2012.8 ID - Caporaso2012 ER - TY - JOUR AU - Schloss, P. D. AU - Westcott, S. L. AU - Ryabin, T. AU - Hall, J. R. AU - Hartmann, M. AU - Hollister, E. B. PY - 2009 DA - 2009// TI - Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities JO - Appl Environ Microbiol VL - 75 UR - https://doi.org/10.1128/AEM.01541-09 DO - 10.1128/AEM.01541-09 ID - Schloss2009 ER - TY - JOUR AU - Quast, C. AU - Pruesse, E. AU - Yilmaz, P. AU - Gerken, J. AU - Schweer, T. AU - Yarza, P. PY - 2013 DA - 2013// TI - The SILVA ribosomal RNA gene database project: improved data processing and web-based tools JO - Nucleic Acids Res VL - 41 UR - https://doi.org/10.1093/nar/gks1219 DO - 10.1093/nar/gks1219 ID - Quast2013 ER - TY - STD TI - Hillman-Jackson J, Clements D, Blankenberg D, Taylor J, Nekrutenko A, Team G. Using galaxy to perform large-scale interactive data analyses. Curr Protoc Bioinform. 2012;38:10–5. ID - ref34 ER - TY - JOUR AU - Shannon, P. AU - Markiel, A. AU - Ozier, O. AU - Baliga, N. S. AU - Wang, J. T. AU - Ramage, D. PY - 2003 DA - 2003// TI - Cytoscape: a software environment for integrated models of biomolecular interaction networks JO - Genome Res Cold Spring Harbor Lab VL - 13 ID - Shannon2003 ER - TY - JOUR AU - Caporaso, J. G. AU - Kuczynski, J. AU - Stombaugh, J. AU - Bittinger, K. AU - Bushman, F. D. AU - Costello, E. K. PY - 2010 DA - 2010// TI - QIIME allows analysis of high-throughput community sequencing data JO - Nat Methods VL - 7 UR - https://doi.org/10.1038/nmeth.f.303 DO - 10.1038/nmeth.f.303 ID - Caporaso2010 ER - TY - STD TI - McDonald D, Price MN, Goodrich J, Nawrocki EP, Desantis TZ, Probst A. An improved Greengenes taxonomy with explicit ranks for ecological and evolutionary analyses of bacteria and archaea. ISME J. 2012;6 doi:10.1038/ismej.2011.139. ID - ref37 ER - TY - JOUR AU - Langille, M. G. I. AU - Zaneveld, J. AU - Caporaso, J. G. AU - McDonald, D. AU - Knights, D. AU - Reyes, J. A. PY - 2013 DA - 2013// TI - Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences JO - Nat Biotechnol VL - 31 UR - https://doi.org/10.1038/nbt.2676 DO - 10.1038/nbt.2676 ID - Langille2013 ER - TY - STD TI - Moissl-Eichinger C. Extremophiles in spacecraft assembly clean rooms. In: Stan-Lotter H, Fendrihan S. (eds) Adaption of Microbial Life to Environmental Extremes. Vienna: Springer; 2012. ID - ref39 ER - TY - JOUR AU - Segata, N. AU - Izard, J. AU - Waldron, L. AU - Gevers, D. AU - Miropolsky, L. AU - Garrett, W. S. PY - 2011 DA - 2011// TI - Metagenomic biomarker discovery and explanation JO - Genome Biol VL - 12 UR - https://doi.org/10.1186/gb-2011-12-6-r60 DO - 10.1186/gb-2011-12-6-r60 ID - Segata2011 ER - TY - JOUR AU - Branquinho, R. AU - Sousa, C. AU - Lopes, J. AU - Pintado, M. E. AU - Peixe, L. V. AU - Osório, H. PY - 2014 DA - 2014// TI - Differentiation of Bacillus Pumilus and bacillus safensis using MALDI-TOF-MS JO - PLoS One VL - 9 UR - https://doi.org/10.1371/journal.pone.0110127 DO - 10.1371/journal.pone.0110127 ID - Branquinho2014 ER - TY - STD TI - Starostin KV, Demidov EA, Bryanskaya AV, Efimov VM, Rozanov AS, Peltek SE. Identification of bacillus strains by MALDI TOF MS using geometric approach. Sci Rep. 2015:5, 16989. ID - ref42 ER - TY - JOUR AU - Hagen, C. A. AU - Hawrylewicz, E. J. AU - Ehrlich, R. PY - 1967 DA - 1967// TI - Survival of microorganisms in a simulated Martian environment. II. Moisture and oxygen requirements for germination of Bacillus Cereus and Bacillus Subtilis Var. niger spores JO - Appl Microbiol VL - 15 ID - Hagen1967 ER - TY - BOOK AU - Hawrylewicz, E. AU - Gowdy, B. AU - Ehrlich, R. PY - 1962 DA - 1962// TI - Micro-organisms under a simulated Martian environment PB - Nature Publishing Group CY - London ID - Hawrylewicz1962 ER - TY - JOUR AU - Imshenetskiĭ, A. A. AU - Murzakov, B. G. AU - Evdokimova, M. D. AU - Dorofeeva, I. K. PY - 1983 DA - 1983// TI - Survival of bacteria in the artificial Mars unit JO - Mikrobiologiia VL - 53 ID - Imshenetskiĭ1983 ER - TY - JOUR AU - Horneck, G. AU - Moeller, R. AU - Cadet, J. AU - Douki, T. AU - Mancinelli, R. L. AU - Nicholson, W. L. PY - 2012 DA - 2012// TI - Resistance of bacterial endospores to outer space for planetary protection purposes—experiment PROTECT of the EXPOSE-E mission JO - Astrobiology VL - 12 UR - https://doi.org/10.1089/ast.2011.0737 DO - 10.1089/ast.2011.0737 ID - Horneck2012 ER - TY - JOUR AU - Fajardo-Cavazos, P. AU - Link, L. AU - Melosh, H. J. AU - Nicholson, W. L. PY - 2005 DA - 2005// TI - Bacillus Subtilis spores on artificial meteorites survive hypervelocity atmospheric entry: implications for lithopanspermia JO - Astrobiology VL - 5 UR - https://doi.org/10.1089/ast.2005.5.726 DO - 10.1089/ast.2005.5.726 ID - Fajardo-Cavazos2005 ER - TY - JOUR AU - Wassmann, M. AU - Moeller, R. AU - Rabbow, E. AU - Panitz, C. AU - Horneck, G. AU - Reitz, G. PY - 2012 DA - 2012// TI - Survival of spores of the UV-resistant Bacillus Subtilis strain MW01 after exposure to low-earth orbit and simulated martian conditions: data from the space experiment ADAPT on EXPOSE-E JO - Astrobiology VL - 12 UR - https://doi.org/10.1089/ast.2011.0772 DO - 10.1089/ast.2011.0772 ID - Wassmann2012 ER - TY - JOUR AU - Hotchin, J. AU - Lorenz, P. AU - Hemenway, C. PY - 1965 DA - 1965// TI - Survival of micro-organisms in space JO - Nature VL - 206 UR - https://doi.org/10.1038/206442a0 DO - 10.1038/206442a0 ID - Hotchin1965 ER - TY - JOUR AU - Horneck, G. AU - Bücker, H. AU - Reitz, G. PY - 1994 DA - 1994// TI - Long-term survival of bacterial spores in space JO - Adv Sp Res Elsevier VL - 14 UR - https://doi.org/10.1016/0273-1177(94)90448-0 DO - 10.1016/0273-1177(94)90448-0 ID - Horneck1994 ER - TY - JOUR AU - Brandstätter, F. AU - Brack, A. AU - Baglioni, P. AU - Cockell, C. S. AU - Demets, R. AU - Edwards, H. G. M. PY - 2008 DA - 2008// TI - Mineralogical alteration of artificial meteorites during atmospheric entry. The STONE-5 experiment. Planet space Sci JO - Elsevier VL - 56 ID - Brandstätter2008 ER - TY - JOUR AU - Zhukova, A. I. AU - Kondratyev, I. I. PY - 1964 DA - 1964// TI - On artificial Martian conditions reproduced for microbiological research JO - Life Sci Space Res VL - 3 ID - Zhukova1964 ER - TY - JOUR AU - Parfenov, G. P. AU - Lukin, A. A. PY - 1973 DA - 1973// TI - Results and prospects of microbiological studies in outer space JO - Space Life Sci Springer VL - 4 ID - Parfenov1973 ER - TY - JOUR AU - von Wintzingerode, F. AU - Rainey, F. A. AU - Kroppenstedt, R. M. AU - Stackebrandt, E. PY - 1997 DA - 1997// TI - Identification of environmental strains of bacillus mycoides by fatty acid analysis and species-specific 16S rDNA oligonucleotide probe JO - FEMS Microbiol Ecol VL - 24 UR - https://doi.org/10.1016/S0168-6496(97)00057-3 DO - 10.1016/S0168-6496(97)00057-3 ID - von Wintzingerode1997 ER - TY - JOUR AU - Zhang, G. AU - Niu, F. AU - Busse, H. -. J. AU - Ma, X. AU - Liu, W. AU - Dong, M. PY - 2008 DA - 2008// TI - Hymenobacter psychrotolerans sp. nov., isolated from the Qinghai—Tibet plateau permafrost region JO - Int J Syst Evol Microbiol VL - 58 UR - https://doi.org/10.1099/ijs.0.65588-0 DO - 10.1099/ijs.0.65588-0 ID - Zhang2008 ER - TY - JOUR AU - Hirsch, P. AU - Ludwig, W. AU - Hethke, C. AU - Sittig, M. AU - Hoffmann, B. AU - Gallikowski, C. A. PY - 1998 DA - 1998// TI - Hymenobacter roseosalivarius gen. Nov., sp. nov. from continental Antarctic soils and sandstone: bacteria of the Cytophaga/Flavobacterium/Bacteroides line of phylogenetic descent JO - Syst Appl Microbiol VL - 21 UR - https://doi.org/10.1016/S0723-2020(98)80047-7 DO - 10.1016/S0723-2020(98)80047-7 ID - Hirsch1998 ER - TY - JOUR AU - Gneiding, K. AU - Frodl, R. AU - Funke, G. PY - 2008 DA - 2008// TI - Identities of microbacterium spp. encountered in human clinical specimens JO - J Clin Microbiol VL - 46 UR - https://doi.org/10.1128/JCM.01202-08 DO - 10.1128/JCM.01202-08 ID - Gneiding2008 ER - TY - JOUR AU - Knief, C. AU - Ramette, A. AU - Frances, L. AU - Alonso-Blanco, C. AU - Vorholt, J. A. PY - 2010 DA - 2010// TI - Site and plant species are important determinants of the methylobacterium community composition in the plant phyllosphere JO - ISME J VL - 4 UR - https://doi.org/10.1038/ismej.2010.9 DO - 10.1038/ismej.2010.9 ID - Knief2010 ER - TY - JOUR AU - Paulino-Lima, I. G. AU - Fujishima, K. AU - Navarrete, J. U. AU - Galante, D. AU - Rodrigues, F. AU - Azua-Bustos, A. PY - 2016 DA - 2016// TI - Extremely high UV-C radiation resistant microorganisms from desert environments with different manganese concentrations JO - J Photochem Photobiol B Biol VL - 163 UR - https://doi.org/10.1016/j.jphotobiol.2016.08.017 DO - 10.1016/j.jphotobiol.2016.08.017 ID - Paulino-Lima2016 ER - TY - JOUR AU - Bashir, M. AU - Ahmed, M. AU - Weinmaier, T. AU - Ciobanu, D. AU - Ivanova, N. AU - Pieber, T. R. PY - 2016 DA - 2016// TI - Functional Metagenomics of spacecraft assembly cleanrooms: presence of virulence factors associated with human pathogens JO - Front Microbiol VL - 7 UR - https://doi.org/10.3389/fmicb.2016.01321 DO - 10.3389/fmicb.2016.01321 ID - Bashir2016 ER - TY - JOUR AU - Salter, S. J. AU - Cox, M. J. AU - Turek, E. M. AU - Calus, S. T. AU - Cookson, W. O. AU - Moffatt, M. F. AU - Turner, P. AU - Parkhill, J. AU - Loman, N. J. AU - Walker, A. W. PY - 2014 DA - 2014// TI - Reagent and laboratory contamination can critically impact sequence-based microbiome analyses JO - BMC Biol VL - 12 UR - https://doi.org/10.1186/s12915-014-0087-z DO - 10.1186/s12915-014-0087-z ID - Salter2014 ER -