Fig. 8From: Interactive effects of temperature, organic carbon, and pipe material on microbiota composition and Legionella pneumophila in hot water plumbing systemsAbsolute number of L. pneumophila quantified by qPCR in comparison to the number of Legionella spp. determined by Illumina sequencing. To determine absolute number of Legionella spp. using Illumina sequencing information, the relative abundance (%) of OTUs belonging to the genera Legionella spp. was multiplied with the number of 16S gene copies quantified qPCRBack to article page