Table 1 Functional classification of glycosyl hydrolases and auxilliary used in this paper based on their characterized catalytic activities according to CAZy (http://www.CAZy.org)
From: Feed in summer, rest in winter: microbial carbon utilization in forest topsoil
Group | Target | GH families |
|---|---|---|
Cellobiose | Cellooligosaccharides | GH1 (β-glucosidase/β-xylosidase), GH3 (β-glucosidase/β-xylosidase/endoxylanase), GH116 (β-glucosidase/β-xylosidase) |
Cellulose | Plant cell wall | GH5_1, GH5_2, GH5_4, GH5_5, GH5_25, GH5_26, GH5_38, GH5_39, GH5_46 (endocellulase), GH6 (exocellulase/endocellulase), GH7 (exocellulase/endocellulase), GH8 (endocellulase/endoxylanase), GH9 (exocellulase/endocellulase/endoxylanase/β-glucosidase), GH12 (endocellulase/endoxylanase), GH44 (endocellulase/endoxylanase), GH45 (endocellulase), GH48 (exocellulase/endocellulase/chitinase), GH74 (endocellulase), AA9 (lytic polysaccharide monooxygenase) |
Chitin | Glucans fungal cell wall | GH5_9, GH5_14, GH5_15 (β-1,3-glucanase/ β-1,6-glucanase), GH16 (endo-1,3-β-glucanase/endo-1,3-β-galactanase), GH17 (endo-1,3-β-glucosidase/ β-1,3-β-glucosidase), GH18 (chitinase), GH19 (chitinase), GH20 (β-hexosaminidase), GH72 (β-1,3-glucanosyltransglycosylase) |
Lignin | Plant cell wall | AA1 (laccase), AA2 (peroxidase), AA3 (oxidase), AA4 (oxidase), AA5 (oxidase), AA6 (oxidase), AA12 (oxidase) |
Pectin | Plant cell wall | GH5_7, GH5_8, GH5_10, GH5_17, GH5_19, GH5_36 (β-mannanase/endo-β-1,4-mannanase), GH28 (polygalacturonase/rhamnogalacturonase), GH62 (α-L-arabinofuranosidase), GH78 (α-Lrhamnosidase), GH88 (β-glucuronyl hydrolase), GH105 (glucuronyl hydrolase/galacturonyl hydrolase), GH106 (α-L-rhamnosidase) |
Peptidoglycan | Bacterial cell wall | GH22 (lysozyme), GH23 (lysozyme), GH24 (lysozyme), GH25 (lysozyme), GH108 (lysozyme) |
Starch/glycogen | Storage compounds | GH13 (amylase/α-glucosidase/trehalase), GH14 (amylase), GH15 (glucoamylase/glucodextranase), GH31 (α-glucosidase), GH57 (amylase), GH77 (amylomaltase), GH119 (amylase) |
Trehalose | Storage compounds | GH37 (trehalase), GH65 (trehalase) |
Xylan | Plant cell wall | GH5_22 (β-xylosidase), GH10 (endo-1,4-β-xylanase/endo-1,3-β-xylanase), GH11 (endoxylanase), GH30 (endoxylanase/β-xylosidase/β-glucosidase), GH67 (xylan α-1,2-glucuronidase), GH115 (xylan α-1,2-glucuronidase), GH120 (β-xylosidase) |