Fig. 1
From: Space-type radiation induces multimodal responses in the mouse gut microbiome and metabolome
Experimental and analytical design. Fecal samples were collected from irradiated mice and processed for both 16S rRNA amplicon and LC-MS profiling. 16S rRNA amplicon data was analyzed at the phylotype level unless stated otherwise. Constrained Analysis of Principal Coordinates (CAP) provided condition-specific phylotypes and metabolites, while model-based clustering produced a classification of highly responsive phylotypes based on overall response to irradiation. The predicted metagenome was employed to estimate contributions of bacterial phylotypes to significant functional shifts and community-wide metabolic potential (CMP) scores. Metabolic network modeling was used to integrate the 16S rRNA amplicon and metabolomics data and to establish significant associations between phylotypes and metabolic shifts