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Fig. 9 | Microbiome

Fig. 9

From: The structure of the Brassica napus seed microbiome is cultivar-dependent and affects the interactions of symbionts and pathogens

Fig. 9

CLSM visualization of bacterial colonization patterns in the untreated (a) and bio-primed with P. brassicacearum CKB26 (b) and S. plymuthica HRO-C48 (c) oilseed rape seeds and in the rhizo- (d) and phyllosphere (e) of the oilseed rape seedlings. The strains in a, b were visualized using either FISH-CLSM using the Alphaproteobacteria-specific ALF968 probe labeled with Alexa488 (blue) and an equimolar ratio of eubacteria probe EUB338, EUB338II, and EUB338III labeled with the fluorescent dye Cy5 (red). In c, BacLight LIVE/DEAD stain was used to visualize alive (green) and dead (red) S. plymuthica HRO-C48 in 3D projection. For the visualization of the P. brassicacearum CKB26 in (d) and (e), the Gammaproteobacteria-specific FISH probe GAM42a labeled with the fluorescent dye Cy5 (green) was overlaid with an equimolar ratio of eubacteria probe EUB338, EUB338II, and EUB338III labeled with the fluorescent dye Cy3 (red). Bacterial colonies are highlighted with arrows. Bar represents 10 μm

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