Fig. 6

Survival rates of Burkholderia spp. inoculation and effects on expression of the citrus ISR-associated genes. Colony-forming units were represented in log10 scale for a strain A53 and b strain A63. Colonies were counted in NA plates supplemented with kanamycin (50 μg/mL) since both strains are kanamycin-resistant. Days post inoculation (dpi) was calculated up to 225, approximately 7 months post inoculation. Error bars represent standard deviation for three biological repeats performed independently. c Relative gene expression of three defense-related citrus genes PR1, PR2, and SAM. Fold change of gene expression is represented at 3, 5, and 7 days post inoculation in root system. Fold change was calculated using ∆∆Ct method. Boxplots represent fold change compared to non-treated plants for three biological replicates. Each biological replicate contains three technical repeats