A novel ultra high-throughput 16S rRNA gene amplicon sequencing library preparation method for the Illumina HiSeq platform

Table 1 ᅟ

Dataset	Sequencer*	Evaluation	Data derived from	Primer list, read numbers and other information
1	MiSeq	PCR1 index	96 mock community samples	Additional file 4: Table S3
2	MiSeq	PCR2 index	20 mock community samples	Additional file 5: Table S4
3	MiSeq	PCR cycle number and input template amount	48 mock community samples	Additional file 6: Table S5
4	HiSeq	HiSeq protocol	24 mock community samples	Additional file 7: Table S6
5	HiSeq	Environmental samples 1	54 standardized fecal samples	Additional file 8: Table S7
6	HiSeq	Environmental samples 2	25 fecal samples from 5 healthy adults	Additional file 9: Table S8
7	MiSeq**, HiSeq	Earth microbiome project	15 fecal samples from 15 healthy adults	Additional file 10: Table S9

*MiSeq - MiSeq v3 600 cycle kit: 300 bp paired end run
*HiSeq - HiSeq 2500 rapid v2 500 cycle kit: 250 bp paired end run
**MiSeq - MiSeq v2 150 cycle kit: 300 bp paired end run
Primer lists, read numbers, and other pertinent information for datasets 1–7 can be found in Additional files 4–10: Tables S3–9, respectively. Environmental samples were DNA extracted using the PowerSoil 96 well DNA isolation kit (MO BIO Laboratories Inc.) and were processed as part of 19 separate sequencing libraries and 2 different HiSeq sequencing runs

ISSN: 2049-2618