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Fig. 7 | Microbiome

Fig. 7

From: A novel ultra high-throughput 16S rRNA gene amplicon sequencing library preparation method for the Illumina HiSeq platform

Fig. 7

Relationship between PCR cycle number and chimeric sequence formation in dataset 3. The combined numbers of PCR1 and PCR2 amplification cycles are indicated on the x-axis. Black and red dots indicate samples amplified using 5 and 10 cycles for PCR2, respectively. A highly significant linear relationship (p < <0.001, linear regression model) was observed. The effects were primarily related to the PCR1 cycle number, e.g., samples undergoing 35 cycles (25 cycle PCR1 and 10 cycles PCR 2) had less chimeras than samples undergoing 35 cycles (30 cycles PCR1 and 5 cycles PCR2)

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