Diet shifts provoke complex and variable changes in the metabolic networks of the ruminal microbiome

Microbiome

Table 2 Diet and network position

Group^a	Currency Cutoff^b	Mean layer: FORG^c	Mean layer: CONC^c	Real Diff.: mean layers^d	Max. Random difference^e	P ^f
VFA	N₂₅	1.99	1.82	0.17	0.006	<0.001
	N₅₀	1.87	1.74	0.13	0.006	<0.001
	N₁₀₀	1.78	1.64	0.14	0.005	<0.001
VFA_AA	N₂₅	0.828	0.66	0.16	0.003	<0.001
	N₅₀	0.87	0.75	0.12	0.004	<0.001
	N₁₀₀	0.91	0.80	0.11	0.004	<0.001
ALL	N₂₅	0.51	0.45	0.06	0.002	<0.001
	N₅₀	0.46	0.41	0.04	0.002	<0.001
	N₁₀₀	0.50	0.47	0.04	0.001	<0.001

^aInterface metabolite set (Materials and Methods)
^bNetwork (e.g., currency cutoff; Materials and Methods)
^cMean layer number for the reads mapped from FORG or CONC animals, respectively.
^dDifference between the mean layer for FORG and CONC
^eMaximum difference in the mean layer for the two diets seen when reads were randomized between the diets
^f P-value for the test of the hypothesis that the two diets do not differ in mean layer. For this test, reads were randomly reassigned to diets and the mean layers recomputed 1000 times (Materials and Methods ). Values significant at P = 0.05 shown in bold.

ISSN: 2049-2618