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Fig. 3 | Microbiome

Fig. 3

From: Optimizing methods and dodging pitfalls in microbiome research

Fig. 3

Wrestling with kit contamination—similar bacterial composition in placental samples and negative controls. Relative abundances of bacterial lineages were inferred from 16S V1-V2 rRNA marker gene sequence information [22]. Samples studied included negative controls, fetal side (FS) placental swabs, maternal side (MS) placental swabs, saliva, and vaginal swabs. Replicates of each sample were extracted using two different kits—the kit type is indicated above each panel. Operating room (OR) air swabs are swabs that were waved in the air at the time of sample collection to be used as negative controls. Saliva samples, which are high in microbial biomass, showed similar compositions for each of the two extractions; placental samples resemble the kit-specific negative controls

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