Fig. 3

a Alpha diversity and b Gene count in relation to mouse strain and diet. Alpha diversity was calculated based on the gene profiles using the Shannon index. In mice fed a low-fat (LF) diet, Sv129 mice exhibited a significantly higher alpha diversity than BL6 mice. High-fat (HF) diet increased alpha diversity of the gut microbiome significantly in both strains of mice, so that no significant differences in alpha diversity were observed after HF feeding. Supplementation with indomethacin did not lead to significant changes of alpha diversity. Gene count in LF-fed Sv129 mice was significantly higher than in BL6 mice. HF feeding led to a significant increase in gene count in both mouse strains and eliminated the difference in gene count observed in LF mice. Statistical differences were analyzed by unpaired Wilcoxon rank-sum test (with FDR correction). Statistically significant differences (P < 0.05) between groups are denoted with different letters (a, b, c, d) on the top of the graphic boxes. Boxes denote the interquartile range (IQR) between the first and third quartiles (25th and 75th percentiles, respectively) and the line inside denotes the median