Fig. 6

Alterations to the functional profiles of microbial communities in adult survivors of childhood ALL by inferred metagenomics. Functional pathways were inferred from OTUs using PICRUSt and annotated with KEGG database. We identified the differently abundant pathways with LEfSe (at LDA threshold of 2) and plotted the LDA scores of pathways of hierarchy level 3 (a). Genes related to the pathways of purine metabolism, DNA repair and recombinant protein and tryptophan metabolism were significantly more abundant in survivors’ microbiome. In contrast, pathways related to bacterial cellular processes and signaling including bacterial motility proteins, sporulation and bacterial chemotaxis were significantly more abundant in controls’ microbiome (b). Data shown as median ± interquartile range and p values was calculated with Mann-Whitney test