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Table 2 Summary of DNA extraction yield and PCR efficiency for extractions performed in phase I, with samples selected for phase II bolded

From: Optimization of DNA extraction for advancing coral microbiota investigations

Treatment × species (number of samples)

Average DNA yield (S.D.) (ng μl−1)a

PCR efficiencyd

PS × P. lobata (3)

4.38 (2.60)b, c

1

PP × P. lobata (3)

3.73 (1.92)b, c

0.67

PB × P. lobata (3)

3.27 (2.86) b, c

1

VGl × P. lobata (3)

0.74 (0.36) c

1

PG × P. lobata (3)

0.40 (0.06) c

0.33

VG × P. lobata (3)

10.31 (5.69) b

0.67

PS × P. verrucosa (3)

7.35 (10.71)

0.67

PP × P. verrucosa (3)

7.96 (7.81)

0

PB × P. verrucosa (3)

27.21 (23.58)

0.67

VGl × P. verrucosa (1)

4.14

1

PG × P. verrucosa (3)

6.06 (3.56)

1

VG × P. verrucosa (3)

12.17 (6.83)

1

PS × A. humilis (3)

18.11 (25.20)

0

PP × A. humilis (3)

7.75 (11.33)

0.33

PB × A. humilis (3)

38.20 (11.70)

1

VGl × A. humilis (2)

9.46 (5.43)

1

PG × A . humilis (3)

5.49 (4.56)

1

VG × A. humilis (3)

29.33 (3.31)

1

PS × O. faveolata (4)

6.51 (2.95)

0.75

PP × O. faveolata (4)

2.24 (1.46)

0

PB × O. faveolata (4)

8.05 (8.08)

1

VGl × O. faveolata (4)

8.24 (7.69)

1

PG × O. faveolata (4)

7.47 (9.79)

1

VG × O. faveolata (4)

12.19 (13.27)

1

PS × M. cavernosa (3)

2.73 (2.10)

0.33

PP × M. cavernosa (3)

1.31 (0.42)

0

PB × M. cavernosa (3)

1.62 (1.06)

0.67

VGl × M. cavernosa (3)

0.67 (0.36)

0.67

PG × M. cavernosa (3)

1.59 (1.58)

1

VG × M. cavernosa (3)

1.79 (1.73)

0.33

PS × O. annularis (3)

7.23 (7.54)

0

PP × O. annularis (3)

1.97 (1.18)

0

PB × O. annularis (3)

9.92 (7.31)

1

VGl × O. annularis (3)

1.63 (1.08)

1

PG × O. annularis (3)

1.47 (1.78)

1

VG × O. annularis (3)

11.47 (13.35)

1

PS × D. strigosa (3)

2.28 (2.25)

0.33

PP × D. strigosa (3)

1.14 (0.29)

0

PB × D. strigosa (3)

0.96 (0.62)

0.33

VGl × D. strigosa (3)

0.24 (0.16)

0.33

PG × D. strigosa (3)

0.33 (0.14)

0.33

VG × D. strigosa (3)

0.98 (0.41)

1

  1. aSuperscripts (b, c) within a column indicate significantly different DNA concentrations between treatments (one-way FRMANOVA; Holm-Sidak method, p < 0.05)
  2. dValues represent normalized PCR efficiency