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Table 1 Comparison of DNA treatment extraction characteristics

From: Optimization of DNA extraction for advancing coral microbiota investigations

MO BIO extraction characteristics

PS

PP

PB

VG

PG

VGl

Bead diameter (mm)

0.7

2.38

0.1, 0.5, 2.4a

0.7

0.1

0.1

Bead type

Garnet

Metal

Glass, Ceramic

Garnet

Glass

Glass

Homogenization method

Vortex

Vortex

Vortex

Vortex

Powerlyzer

Vortex

Homogenization duration

15 min

15 min

15 min

15 min

45 s

15 min

Surfactant

<5%

<5%

X

X

X

X

Protein precipitant

20–40%

10–20%

10–15%

X

X

X

Guanidine thiocyanate

X

<3%

<3%, 60–80%

X

X

X

Inhibitor removal

<10%

<5%

<10%

X

X

X

Proteinase K

X

X

X

1–5%

1–5%

1–5%

RNase

X

25%

X

X

X

X

Phenolic separation solution

X

5–15%

X

X

X

X

  1. X indicates that the parameter was not included
  2. PS PowerSoil, PP PowerPlant Pro, PB PowerBiofilm, VG UC Vortex Garnet, PG UC Powerlyzer Glass, VGl UC Vortex Glass
  3. aPB kit uses 0.1- and 0.5-mm glass beads and 2.4-mm ceramic beads