From: Optimization of DNA extraction for advancing coral microbiota investigations
MO BIO extraction characteristics | PS | PP | PB | VG | PG | VGl |
---|---|---|---|---|---|---|
Bead diameter (mm) | 0.7 | 2.38 | 0.1, 0.5, 2.4a | 0.7 | 0.1 | 0.1 |
Bead type | Garnet | Metal | Glass, Ceramic | Garnet | Glass | Glass |
Homogenization method | Vortex | Vortex | Vortex | Vortex | Powerlyzer | Vortex |
Homogenization duration | 15Â min | 15Â min | 15Â min | 15Â min | 45Â s | 15Â min |
Surfactant | <5% | <5% | X | X | X | X |
Protein precipitant | 20–40% | 10–20% | 10–15% | X | X | X |
Guanidine thiocyanate | X | <3% | <3%, 60–80% | X | X | X |
Inhibitor removal | <10% | <5% | <10% | X | X | X |
Proteinase K | X | X | X | 1–5% | 1–5% | 1–5% |
RNase | X | 25% | X | X | X | X |
Phenolic separation solution | X | 5–15% | X | X | X | X |