| Â | Experimental soil | Natural sitea
| Climate chamber |
|---|
Sample | – | Plot 1 | Plot 2 | Plot 3 | Exb 1 | Ex 2c
| Ex 3 | Ex 4 | Ex 5c
| Microcosms |
|---|
Root | – | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 8d/12e
|
Soil | 3 | 3 | 3 | 3 | 3 | – | – | – | – | 3 |
Inoculum | – | – | – | – | – | – | – | – | – | 4f/3g
|
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aBacteria isolates from natural site plants were cultured from plants collected from within and outside the experimental plots
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bExperiment
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cBacteria isolates from climate chamber plants were cultured from these experiments, plus one non-sequenced growth experiment
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dTotal number of samples collected from the soil extract experiment. One root sample was collected from each replicate microcosm
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eTotal number of samples from the simplified community experiments. Four root samples were collected from each of the three experiments
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fIndependently prepared soil extract samples used as the experimental start inoculum. See Additional file 1 for details
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gOne inoculum sample for each microcosm experiment
