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Fig. 1 | Microbiome

Fig. 1

From: Deciphering composition and function of the root microbiome of a legume plant

Fig. 1

Characterization of the root microbiome. We collected a natural field soil and used it in a series of Trifolium growth experiments. (I) We investigated the composition of the root bacteria microbiome using 16S rRNA sequencing of root samples. (II) We utilized the same root material for an isolation effort to explore the culturable fraction of root bacteria microbiome and assembled a reference stock of bacteria isolates. (III) We subsequently developed a microcosm system to explore plant-microbiota interactions and (IV) investigated the composition of the Trifolium root microbiome in the system by inoculating microbiota extracted from the field soil. (V) We conducted microbiota manipulation experiments in which we inoculated culturable, abundant members of the root microbiome and scored their effects on plant growth

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