A fast and robust protocol for metataxonomic analysis using RNAseq data

Microbiome

Table 3 Average classification performance by metagenome database used

Database	Bacteria		Bacteria		Fungi		Fungi		Virus
	Species level		Genus level		Species level		Genus level		First taxon level
	TPR	FDR	TPR	FDR	TPR	FDR	TPR	FDR	TPR	FDR
RefSeq	0.76 ± 0.12	0.56 ± 0.20	0.83 ± 0.13	0.34 ± 0.19	0.78 ± 0.05	0.79 ± 0.19	0.89 ± 0.08	0.72 ± 0.23	0.95 ± 0.14	0.05 ± 0.07
Custom	0.78 ± 0.12	0.34 ± 0.12	0.84 ± 0.13	0.07 ± 0.07	0.98 ± 0.05	0.45 ± 0.21	0.96 ± 0.08	0.41 ± 0.19	0.99 ± 0.03	0.08 ± 0.10
p value	0.017	<0.001	0.507	<0.001	<0.001	<0.001	<0.001	<0.001	0.353	0.378

TPR and FDR are averaged across 18 experiments each, statistically significant results highlighted in bold

ISSN: 2049-2618