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Table 1 Simulated datasets used for evaluating and optimizing the IMSA+A protocol

From: A fast and robust protocol for metataxonomic analysis using RNAseq data

Experiment

  

Parameters used to vary coverage

Other parameters controlled for this experiment

        

Read length/coverage

Read length

Bacteria Coverage

Bacteria seq depthb

Bacteria gene selection

Bacteria speciesa

Fungi coverage

Fungi seq depthb

Fungi gene selection

Fungi speciesa

Virus coverage

Virus seq depthb

Virus gene selection

Virus strainsa

Human coverage

Human gene coverage

Human Seq depthb

Human reads after subtraction

50 low

50

0.25

1.0

100%

30

1.1

2.4

50%

15

16.2

0.1

100%

10

14.19

100%

66.5

0.078

50 med

50

1.11

1.0

25%

30

1.1

2.4

50%

15

16.2

0.1

100%

10

14.19

100%

66.5

0.078

50 high

50

4.44

4.0

25%

30

1.1

2.4

50%

15

16.2

0.1

100%

10

14.19

100%

66.5

0.078

100 low

100

0.87

1.0

100%

30

2.3

2.4

50%

15

106.4

0.1

100%

10

28.31

100%

66.5

0.074

100 med

100

2.22

1.0

25%

30

2.3

2.4

50%

15

106.4

0.1

100%

10

28.31

100%

66.5

0.074

100 high

100

8.88

4.0

25%

30

2.3

2.4

50%

15

106.4

0.1

100%

10

28.31

100%

66.5

0.074

150 low

150

1.30

1.0

100%

30

3.4

2.4

50%

15

159.5

0.1

100%

10

42.46

100%

66.5

0.054

150 med

150

3.33

1.0

25%

30

3.4

2.4

50%

15

159.5

0.1

100%

10

42.46

100%

66.5

0.054

150 high

150

13.33

4.0

25%

30

3.4

2.4

50%

15

159.5

0.1

100%

10

42.46

100%

66.5

0.054

  1. aSimulated organisms were the same across experiments as an experimental control
  2. bSequencing depth in millions