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Table 1 List of used media and conditions

From: Resilient microorganisms in dust samples of the International Space Station—survival of the adaptation specialists

Medium Phase pH Incubation temperature Gasphase Abbreviation Target organisms Medium reference
R2A agar pH 5 Solid 5 30 °C Aerobic (ambient) pH 5 Acidophiles –
R2A agar pH 9 Solid 9 30 °C Aerobic (ambient) pH 9 Alkaliphiles –
R2A agar pH 7 Solid 7 30 °C Aerobic (ambient) pH 7F Heterotrophs –
RAVAN pH 7 for oligotrophs Solid 7 30 °C Aerobic (ambient) RAV Oligotrophs [72] (modifieda)
DSMZ_Medium97 for halophiles Liquid 7.5 40 °C Aerobic (ambient) Halo Halophiles DSM 97 (www.dsmz.de)
R2A pH 7 liquid Liquid 7 30 °C N2 pH 7an Anaerobes –
Medium for methanogens Liquid 7 40 °C H2CO2 (80:20) MS Methanogens [73]
MS supplemented with 0.1% yeast extract and 0.1% acetate Liquid 7 40 °C H2CO2 (80:20) MS_sup Methanogens –
Archaea-supporting liquid medium Liquid 7 30 °C N2 ASM Archaea [74]b
ASM supplemented with 0.1% yeast extract and 0.1% acetate Liquid 7 30 °C N2 ASM_sup Archaea –
Autotrophic all-rounder liquid medium Liquid 7 30 °C N2CO2 (80:20) AAM Autotrophs [74]b
Autotrophic homoacetogen liquid medium Liquid 7.5 30 °C H2CO2 (80:20) AHM Autotrophs [74]b
  1. a1:100 diluted, final concentration of 50 mg/l sodium pyruvate instead of 20 mg/l pyruvic acid
  2. bWithout addition of antibiotics
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Microbiome

ISSN: 2049-2618

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