Mungo bean sprout microbiome and changes associated with culture based enrichment protocols used in detection of Gram-negative foodborne pathogens

Table 3 Proportions of differentially abundant phyla and genera in selected groups using Metastats analysis (P ≤ 0.05). Samples before enrichment (t = 0) and samples after enrichment in either BPW or EE-broth for 16 h were compared

Genera	Phyla	t = 0	BPW 37 °C (16 h)	P value
	Firmicutes	0.057 ± 0.0001	0.092 ± 0.0003	0.008
Acinetobacter		0.032 ± 0.001	0.008 ± 0.00007	0.043
Duganella		0.049 ± 0.0004	0	0.005
Pseudomonas		0.151 ± 0.004	0.015 ± 0.0006	0.024
		t = 0	BPW 42 °C (16 h)
	Firmicutes	0.002 ± 0.000008	0.207 ± 0.023	0.337
Duganella		0.024 ± 0.0001	0	0.005
Pseudomonas		0.074 ± 0.0009	0.009 ± 0.00006	0.014
		t = 0	EE 37 °C (16 h)
	Bacteroidetes	0.046 ± 0.001	0.002 ± 0.000008	0.002
Duganella		0.024 ± 0.001	0.002 ± 0.000008	0.041
Kosakonia		0.027 ± 0.005	0.002 ± 0.000008	0.046
Pseudomonas		0.074 ± 0.0009	0.007 ± 0.0001	0.024
		t = 0	EE 42 °C (16 h)
	Bacteroidetes	0.046 ± 0.001	0.002 ± 0.000008	0.045
Duganella		0.024 ± 0.0001	0	0.018
Pseudomonas		0.074 ± 0.0009	0.007 ± 0.00005	0.023
		BPW 37 °C (16 h)	EE 37 °C (16 h)
	Firmicutes	0.231 ± 0.018	0.0003 ± 0.0001	0.021
	Proteobacteria	0.286 ± 0.017	0.499 ± 0.0000005	0.015
Acinetobacter		0.039 ± 0.0000009	0.0002 ± 0.00000004	0
Kosakonia		0.018 ± 0.00005	0.002 ± 0.000005	0.01
		BPW 37 °C (16 h)	BPW 42 °C (16 h)
Kosakonia		0.018 ± 0.00008	0.002 ± 0.0000005	0.028

ISSN: 2049-2618