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Table 1 Summary of study experiments

From: Latitude in sample handling and storage for infant faecal microbiota studies: the elephant in the room?

Experiment Subject population Faecal sample source DNA extraction protocol Sequencing region Sequencing platform
Variation between extractions Term infants Infant 14 2 Vv4 Illumina Miseq
Effects of varied weight Premature infants Infants 1, 2, 3 and 4 2 V3–V5 Roche 454 GS FLX
Effects of long term freezing Premature infants Infants 5, 6, 7 and 8 1 V4 Illumina Miseq
Effects of room temperature storage Premature and term infants Infants 5, 6, 7, 8, 9, 10, 11, 12 and 13 2 V4 Illumina Miseq
Effects of mailing samples Term infants Infants 9, 10, 11, 12 and 13 2 V4 Illumina Miseq
  1. Experiments performed to determine the effects of different sources of variation. Term infant faecal communities represent more complex microbial mixtures, whilst premature faecal samples harbour more simple communities and are obtained in very limited quantities