Table 1 Summary of study experiments
Experiment | Subject population | Faecal sample source | DNA extraction protocol | Sequencing region | Sequencing platform |
|---|---|---|---|---|---|
Variation between extractions | Term infants | Infant 14 | 2 | Vv4 | Illumina Miseq |
Effects of varied weight | Premature infants | Infants 1, 2, 3 and 4 | 2 | V3–V5 | Roche 454 GS FLX |
Effects of long term freezing | Premature infants | Infants 5, 6, 7 and 8 | 1 | V4 | Illumina Miseq |
Effects of room temperature storage | Premature and term infants | Infants 5, 6, 7, 8, 9, 10, 11, 12 and 13 | 2 | V4 | Illumina Miseq |
Effects of mailing samples | Term infants | Infants 9, 10, 11, 12 and 13 | 2 | V4 | Illumina Miseq |