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Fig. 1 | Microbiome

Fig. 1

From: Latitude in sample handling and storage for infant faecal microbiota studies: the elephant in the room?

Fig. 1

PCoA plots of four beta diversity measures (panels a-d; a - Weighted UniFrac, b - Unweighted Unifrac, c - Bray-Curtis dissimilarity, d - Jaccard dissimilarity), displaying results from two experiments: (i) red and blue points indicate the samples used for the DNA extraction similarity experiment, where a single faecal sample from a term infant (infant 14) was split into ten aliquots. Samples are grouped according which the DNA extraction they were processed in; each group underwent the same protocol, but was processed by a different researcher, using FastDNA SPIN Kits for soil (MP Biomedicals) with different lot numbers. Samples were frozen at −80 °C prior to extraction and processed after 24 days (DNA extraction 1, red) or 115 days (DNA extraction 2, blue), simulating typical staggered collection and DNA extraction of samples. (ii) Orange, green, purple and yellow points indicate samples included in the long-term storage experiment, where samples from four infants were split into aliquots and stored at −80 °C for short-term (2 months) storage or at −80 °C for ‘long-term’ (6, 12, 18 and 24 months) storage prior to DNA extraction. Points are coloured by infant and labelled with the length of storage

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