Fig. 1From: A comprehensive method for amplicon-based and metagenomic characterization of viruses, bacteria, and eukaryotes in freshwater samplesGene copy numbers of 16S rRNA (a), uidA (b), β-tubulin (c), and g23 (d) gene fragments detected in watershed sites. UPL urban polluted, UDS urban downstream, AUP agricultural upstream site, APL agricultural polluted, ADS agricultural downstream, PUP protected upstream, PDS protected downstream. Black bars represent the mean GCN normalized per nanogram of DNA in each location (n = 3). Gray bars represent the mean GCN normalized per milliliter of sample (n = 3). Error bars indicate standard deviations. Means with different letters indicate statistical significance between watershed sites at the 0.05 levelBack to article page