Fig. 7
From: Colitis susceptibility in p47phox−/− mice is mediated by the microbiome
Homogenizing the intestinal microbiome at birth decreases DSS colitis susceptibility in p47phox−/− mice. p47phox+/− mice were bred, and weanlings were placed in separate cages by gender and genotype (p47phox−/− versus B6Tac) prior to DSS colitis induction. Changes in body weight (a), disease activity index (b), and survival (c) were assessed daily (B6Tac (n = 7); p47phox−/− (n = 8)). Significance was determined using the Mann-Whitney U test (*p < 0.05) and a log-rank test for survival (p = 0.17). d Colonic tissue sections were blindly scored for inflammation, depth of injury, and crypt damage on day 9 (B6Tac (n = 7); p47phox−/− (n = 6); (p = 0.619)). e Bacterial translocation to MLNs was determined on day 9. The number of CFUs per MLN is shown (B6Tac (n = 7); p47phox−/− (n = 6)); (p = 0.346)). Pie charts represent the proportion of mice per group where the cultured MLN grew one or more of the listed bacterial species. For all panels, data were generated from 2 independent experiments (except E). f Plasma was collected on day 9 for cytokine measurement; p values <0.05 are indicated