Fig. 2From: Optimizing protocols for extraction of bacteriophages prior to metagenomic analyses of phage communities in the human gutBacteriophage recovery monitored in LIT (a), PEG (b), and TFF (c) protocols. The fecal samples were spiked with a set of bacteriophages, c2, ϕ29, and T4, and used as a process control for protocol optimization. The population of bacteriophages was monitored at each step by plaque assay (plaque-forming units (PFU)). Reductions in the curves of phage population indicate the degree of loss at a given stepBack to article page